Toluene biodegradation in the vadose zone of a poplar phytoremediation system identified using metagenomics and toluene-specific stable carbon isotope analysis.
Identifieur interne : 000661 ( Main/Exploration ); précédent : 000660; suivant : 000662Toluene biodegradation in the vadose zone of a poplar phytoremediation system identified using metagenomics and toluene-specific stable carbon isotope analysis.
Auteurs : Michael Benisrael [Canada] ; Philipp Wanner [Canada] ; Ramon Aravena [Canada] ; Beth L. Parker [Canada] ; Elizabeth A. Haack [Canada] ; David T. Tsao [États-Unis] ; Kari E. Dunfield [Canada]Source :
- International journal of phytoremediation [ 1549-7879 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Toluene.
- chemical : Carbon, Carbon Isotopes, RNA, Ribosomal, 16S, Water Pollutants, Chemical.
- Biodegradation, Environmental, Groundwater, Metagenomics.
Abstract
Biodegradation is an important mechanism of action of phytoremediation systems, but performance evaluation is challenging. We applied metagenomic molecular approaches and compound-specific stable carbon isotope analysis to assess biodegradation of toluene in the vadose zone at an urban pilot field system where hybrid poplars were planted to remediate legacy impacts to an underlying shallow fractured bedrock aquifer. Carbon isotope ratios were compared spatio-temporally between toluene dissolved in groundwater and in the vapor phase. Enrichment of 13C from toluene in the vapor phase compared to groundwater provided evidence for biodegradation in the vadose zone. Total bacterial abundance (16S rRNA) and abundance and expression of degradation genes were determined in rhizosphere soil (DNA and RNA) and roots (DNA) using quantitative PCR. Relative abundances of degraders in the rhizosphere were on average higher at greater depths, except for enrichment of PHE-encoding communities that more strongly followed patterns of toluene concentrations detected. Quantification of RMO and PHE gene transcripts supported observations of active aerobic toluene degradation. Finally, spatially-variable numbers of toluene degraders were detected in poplar roots. We present multiple lines of evidence for biodegradation in the vadose zone at this site, contributing to our understanding of mechanisms of action of the phytoremediation system.
DOI: 10.1080/15226514.2018.1523873
PubMed: 30648419
Affiliations:
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Parker</name><affiliation wicri:level="1"><nlm:affiliation>b G360 Institute for Groundwater Research , University of Guelph , Guelph , Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>b G360 Institute for Groundwater Research , University of Guelph , Guelph </wicri:regionArea><wicri:noRegion>Guelph </wicri:noRegion></affiliation></author><author><name sortKey="Haack, Elizabeth A" sort="Haack, Elizabeth A" uniqKey="Haack E" first="Elizabeth A" last="Haack">Elizabeth A. Haack</name><affiliation wicri:level="1"><nlm:affiliation>d EcoMetrix Inc , Mississauga , Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>d EcoMetrix Inc , Mississauga </wicri:regionArea><wicri:noRegion>Mississauga </wicri:noRegion></affiliation></author><author><name sortKey="Tsao, David T" sort="Tsao, David T" uniqKey="Tsao D" first="David T" last="Tsao">David T. Tsao</name><affiliation wicri:level="1"><nlm:affiliation>e BP Corporation North America Inc , Naperville , USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>e BP Corporation North America Inc , Naperville </wicri:regionArea><wicri:noRegion>Naperville </wicri:noRegion></affiliation></author><author><name sortKey="Dunfield, Kari E" sort="Dunfield, Kari E" uniqKey="Dunfield K" first="Kari E" last="Dunfield">Kari E. Dunfield</name><affiliation wicri:level="1"><nlm:affiliation>a School of Environmental Sciences , University of Guelph , Guelph , Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>a School of Environmental Sciences , University of Guelph , Guelph </wicri:regionArea><wicri:noRegion>Guelph </wicri:noRegion></affiliation></author></analytic><series><title level="j">International journal of phytoremediation</title><idno type="eISSN">1549-7879</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodegradation, Environmental (MeSH)</term><term>Carbon (MeSH)</term><term>Carbon Isotopes (MeSH)</term><term>Groundwater (MeSH)</term><term>Metagenomics (MeSH)</term><term>RNA, Ribosomal, 16S (MeSH)</term><term>Toluene (analysis)</term><term>Water Pollutants, Chemical (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN ribosomique 16S (MeSH)</term><term>Carbone (MeSH)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Isotopes du carbone (MeSH)</term><term>Métagénomique (MeSH)</term><term>Nappe phréatique (MeSH)</term><term>Polluants chimiques de l'eau (MeSH)</term><term>Toluène (analyse)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Toluene</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Carbon</term><term>Carbon Isotopes</term><term>RNA, Ribosomal, 16S</term><term>Water Pollutants, Chemical</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Toluène</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Groundwater</term><term>Metagenomics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN ribosomique 16S</term><term>Carbone</term><term>Dépollution biologique de l'environnement</term><term>Isotopes du carbone</term><term>Métagénomique</term><term>Nappe phréatique</term><term>Polluants chimiques de l'eau</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Biodegradation is an important mechanism of action of phytoremediation systems, but performance evaluation is challenging. We applied metagenomic molecular approaches and compound-specific stable carbon isotope analysis to assess biodegradation of toluene in the vadose zone at an urban pilot field system where hybrid poplars were planted to remediate legacy impacts to an underlying shallow fractured bedrock aquifer. Carbon isotope ratios were compared spatio-temporally between toluene dissolved in groundwater and in the vapor phase. Enrichment of <sup>13</sup>C from toluene in the vapor phase compared to groundwater provided evidence for biodegradation in the vadose zone. Total bacterial abundance (16S rRNA) and abundance and expression of degradation genes were determined in rhizosphere soil (DNA and RNA) and roots (DNA) using quantitative PCR. Relative abundances of degraders in the rhizosphere were on average higher at greater depths, except for enrichment of PHE-encoding communities that more strongly followed patterns of toluene concentrations detected. Quantification of RMO and PHE gene transcripts supported observations of active aerobic toluene degradation. Finally, spatially-variable numbers of toluene degraders were detected in poplar roots. We present multiple lines of evidence for biodegradation in the vadose zone at this site, contributing to our understanding of mechanisms of action of the phytoremediation system.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">30648419</PMID><DateCompleted><Year>2019</Year><Month>09</Month><Day>05</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1549-7879</ISSN><JournalIssue CitedMedium="Internet"><Volume>21</Volume><Issue>1</Issue><PubDate><Year>2019</Year></PubDate></JournalIssue><Title>International journal of phytoremediation</Title><ISOAbbreviation>Int J Phytoremediation</ISOAbbreviation></Journal><ArticleTitle>Toluene biodegradation in the vadose zone of a poplar phytoremediation system identified using metagenomics and toluene-specific stable carbon isotope analysis.</ArticleTitle><Pagination><MedlinePgn>60-69</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1080/15226514.2018.1523873</ELocationID><Abstract><AbstractText>Biodegradation is an important mechanism of action of phytoremediation systems, but performance evaluation is challenging. We applied metagenomic molecular approaches and compound-specific stable carbon isotope analysis to assess biodegradation of toluene in the vadose zone at an urban pilot field system where hybrid poplars were planted to remediate legacy impacts to an underlying shallow fractured bedrock aquifer. Carbon isotope ratios were compared spatio-temporally between toluene dissolved in groundwater and in the vapor phase. Enrichment of <sup>13</sup>C from toluene in the vapor phase compared to groundwater provided evidence for biodegradation in the vadose zone. Total bacterial abundance (16S rRNA) and abundance and expression of degradation genes were determined in rhizosphere soil (DNA and RNA) and roots (DNA) using quantitative PCR. Relative abundances of degraders in the rhizosphere were on average higher at greater depths, except for enrichment of PHE-encoding communities that more strongly followed patterns of toluene concentrations detected. Quantification of RMO and PHE gene transcripts supported observations of active aerobic toluene degradation. Finally, spatially-variable numbers of toluene degraders were detected in poplar roots. We present multiple lines of evidence for biodegradation in the vadose zone at this site, contributing to our understanding of mechanisms of action of the phytoremediation system.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>BenIsrael</LastName><ForeName>Michael</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>a School of Environmental Sciences , University of Guelph , Guelph , Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wanner</LastName><ForeName>Philipp</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>b G360 Institute for Groundwater Research , University of Guelph , Guelph , Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aravena</LastName><ForeName>Ramon</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>b G360 Institute for Groundwater Research , University of Guelph , Guelph , Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>c Department of Earth and Environmental Sciences , University of Waterloo , Waterloo , Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Parker</LastName><ForeName>Beth L</ForeName><Initials>BL</Initials><AffiliationInfo><Affiliation>b G360 Institute for Groundwater Research , University of Guelph , Guelph , Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Haack</LastName><ForeName>Elizabeth A</ForeName><Initials>EA</Initials><AffiliationInfo><Affiliation>d EcoMetrix Inc , Mississauga , Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tsao</LastName><ForeName>David T</ForeName><Initials>DT</Initials><AffiliationInfo><Affiliation>e BP Corporation North America Inc , Naperville , USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dunfield</LastName><ForeName>Kari E</ForeName><Initials>KE</Initials><AffiliationInfo><Affiliation>a School of Environmental Sciences , University of Guelph , Guelph , Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>01</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Int J Phytoremediation</MedlineTA><NlmUniqueID>101136878</NlmUniqueID><ISSNLinking>1522-6514</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002247">Carbon Isotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014874">Water Pollutants, Chemical</NameOfSubstance></Chemical><Chemical><RegistryNumber>3FPU23BG52</RegistryNumber><NameOfSubstance UI="D014050">Toluene</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-44-0</RegistryNumber><NameOfSubstance UI="D002244">Carbon</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002247" MajorTopicYN="N">Carbon Isotopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060587" MajorTopicYN="Y">Groundwater</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D056186" MajorTopicYN="N">Metagenomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012336" MajorTopicYN="N">RNA, Ribosomal, 16S</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014050" MajorTopicYN="N">Toluene</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014874" MajorTopicYN="Y">Water Pollutants, Chemical</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">BTEX</Keyword><Keyword MajorTopicYN="N">CSIA</Keyword><Keyword MajorTopicYN="N">gene expression</Keyword><Keyword MajorTopicYN="N">qPCR</Keyword><Keyword MajorTopicYN="N">rhizosphere</Keyword><Keyword MajorTopicYN="N">root microbiome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>1</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>9</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>1</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30648419</ArticleId><ArticleId IdType="doi">10.1080/15226514.2018.1523873</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>États-Unis</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Benisrael, Michael" sort="Benisrael, Michael" uniqKey="Benisrael M" first="Michael" last="Benisrael">Michael Benisrael</name></noRegion><name sortKey="Aravena, Ramon" sort="Aravena, Ramon" uniqKey="Aravena R" first="Ramon" last="Aravena">Ramon Aravena</name><name sortKey="Aravena, Ramon" sort="Aravena, Ramon" uniqKey="Aravena R" first="Ramon" last="Aravena">Ramon Aravena</name><name sortKey="Dunfield, Kari E" sort="Dunfield, Kari E" uniqKey="Dunfield K" first="Kari E" last="Dunfield">Kari E. Dunfield</name><name sortKey="Haack, Elizabeth A" sort="Haack, Elizabeth A" uniqKey="Haack E" first="Elizabeth A" last="Haack">Elizabeth A. Haack</name><name sortKey="Parker, Beth L" sort="Parker, Beth L" uniqKey="Parker B" first="Beth L" last="Parker">Beth L. Parker</name><name sortKey="Wanner, Philipp" sort="Wanner, Philipp" uniqKey="Wanner P" first="Philipp" last="Wanner">Philipp Wanner</name></country><country name="États-Unis"><noRegion><name sortKey="Tsao, David T" sort="Tsao, David T" uniqKey="Tsao D" first="David T" last="Tsao">David T. Tsao</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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